Prof. Dr. Aljoscha Wahl

Professur für Systembiotechnologie

Department Chemie- und Bioingenieurwesen (CBI)
Lehrstuhl für Bioverfahrenstechnik (BVT)

Raum 00.040
Konrad-Zuse-Str. 3
91052 Erlangen

Postadresse:
Paul-Gordan-Str. 3
91052 Erlangen

Aktuelle Position:

W2-Professor für Systembiotechnologie

seit 01/2022

Lehrstuhl für Bioverfahrenstechnik, FAU Erlangen-Nürnberg

Kurz-Lebenslauf:

Assistant Professor	2008 – 2021	TU Delft, Department of Biotechnology, Niederlande Link zur Webseite von S. A. Wahl
Postdoktorand	2006 – 2008	Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany, Bioprocess Engineering group, Prof. U. Reichl
Doktorarbeit	2007	„Methoden zur integrierten Analyse metabolischer Netzwerke unter stationären und instationären Bedingungen“ (Betreuung durch Prof. Wiechert, FZ Jülich)
wiss. Mitarbeiter	2003 – 2006	Institut für Biotechnologie 2, Forschungszentrum Jülich, Jülich – Prof. Wandrey und Prof. Wiechert
Diplom	1997 – 2003	Studium der Bioverfahrenstechnik, TU Dresden

Editorial Board Memberships:

seit 2016	Scientific Reports
seit 2010	Applied and Environmental Microbiology (two terms)

Guest Editor – special edition:

Special Issue „Metabolic Functionality of Microorganisms under Dynamic Environments“

Publikationen:

2023

Lao-Martil D., Verhagen KJ., Valdeira Caetano AH., Pardijs IH., van Riel NA., Wahl SA.:
Using Kinetic Modelling to Infer Adaptations in Saccharomyces cerevisiae Carbohydrate Storage Metabolism to Dynamic Substrate Conditions
In: Metabolites 13 (2023), Art.Nr.: 88
ISSN: 2218-1989
DOI: 10.3390/metabo13010088
Soares Rodrigues CI., den Ridder M., Pabst M., Gombert AK., Wahl SA.:
Comparative proteome analysis of different Saccharomyces cerevisiae strains during growth on sucrose and glucose
In: Scientific Reports 13 (2023), Art.Nr.: 2126
ISSN: 2045-2322
DOI: 10.1038/s41598-023-29172-0

2022

Gallego-Murillo JS., Iacono G., van der Wielen LA., van den Akker E., von Lindern M., Wahl SA.:
Expansion and differentiation of ex vivo cultured erythroblasts in scalable stirred bioreactors
In: Biotechnology and Bioengineering (2022)
ISSN: 0006-3592
DOI: 10.1002/bit.28193
Lao-Martil D., Verhagen KJA., Schmitz JP., Teusink B., Wahl SA., van Riel NA.:
Kinetic Modeling of Saccharomyces cerevisiae Central Carbon Metabolism: Achievements, Limitations, and Opportunities
In: Metabolites 12 (2022), Art.Nr.: 74
ISSN: 2218-1989
DOI: 10.3390/metabo12010074
Verhagen KJA., Eerden SA., Sikkema BJ., Wahl SA.:
Predicting Metabolic Adaptation Under Dynamic Substrate Conditions Using a Resource-Dependent Kinetic Model: A Case Study Using Saccharomyces cerevisiae
In: Frontiers in Molecular Biosciences 9 (2022), Art.Nr.: 863470
ISSN: 2296-889X
DOI: 10.3389/fmolb.2022.863470

2021

Evers B., Gerding A., Boer T., Heiner-Fokkema MR., Jalving M., Reijngoud DJ., Bakker BM., Wahl SA.:
Simultaneous quantification of the concentration and carbon isotopologue distribution of polar metabolites in a single analysis by gas chromatography and mass spectrometry
In: Analytical Chemistry 93 (2021), S. 8248-8256
ISSN: 0003-2700
DOI: 10.1021/acs.analchem.1c01040
Mcdaniel EA., Wahl SA., Ishii SI., Pinto A., Ziels R., Nielsen PH., Mcmahon KD., Williams RBH.:
Prospects for multi-omics in the microbial ecology of water engineering
In: Water Research 205 (2021), Art.Nr.: 117608
ISSN: 0043-1354
DOI: 10.1016/j.watres.2021.117608
Olavarria K., Carnet A., Van Renselaar J., Quakkelaar C., Cabrera R., Da Silva LG., Smids AL., Andres Villalobos P., Van Loosdrecht MCM., Wahl SA.:
An NADH preferring acetoacetyl-CoA reductase is engaged in poly-3-hydroxybutyrate accumulation in Escherichia coli
In: Journal of Biotechnology 325 (2021), S. 207-216
ISSN: 0168-1656
DOI: 10.1016/j.jbiotec.2020.10.022
Regueira A., Rombouts JL., Mauricio-Iglesias M., Lema JM., Kleerebezem R., Wahl SA.:
Resource allocation explains lactic acid production in mixed-culture anaerobic fermentations
In: Biotechnology and bioengineering 118 (2021), S. 745-758
ISSN: 1097-0290
DOI: 10.1002/bit.27605
Soares Rodrigues CI., Gombert AK., Wahl SA.:
Aerobic growth physiology of Saccharomyces cerevisiae on sucrose is strain-dependent
In: Fems Yeast Research 21 (2021), Art.Nr.: foab021
ISSN: 1567-1356
DOI: 10.1093/femsyr/foab021

2020

Vasilakou E., Van Loosdrecht MCM., Wahl SA.:
Escherichia coli metabolism under short-Term repetitive substrate dynamics: Adaptation and trade-offs
In: Microbial Cell Factories 19 (2020), Art.Nr.: 116
ISSN: 1475-2859
DOI: 10.1186/s12934-020-01379-0
Verhagen KJA., Van Gulik WM., Wahl SA.:
Dynamics in redox metabolism, from stoichiometry towards kinetics
In: Current Opinion in Biotechnology 64 (2020), S. 116-123
ISSN: 0958-1669
DOI: 10.1016/j.copbio.2020.01.002
Zhang J., Van Den Herik BM., Wahl SA.:
Alpha-ketoglutarate utilization in Saccharomyces cerevisiae: transport, compartmentation and catabolism
In: Scientific Reports 10 (2020), Art.Nr.: 12838
ISSN: 2045-2322
DOI: 10.1038/s41598-020-69178-6
da Silva LG., Gamez KO., Gomes JC., Akkermans K., Welles L., Abbas B., Loosdrecht MC., Wahl SA.:
Revealing the Metabolic Flexibility of “Candidatus Accumulibacter phosphatis” through Redox Cofactor Analysis and Metabolic Network Modeling
In: Applied and Environmental Microbiology 86 (2020), S. 1-17
ISSN: 0099-2240
DOI: 10.1128/AEM.0808-20

2019

Doerr A., De Reus E., Van Nies P., Van Der Haar M., Wei K., Kattan J., Danelon C., Wahl SA.:
Modelling cell-free RNA and protein synthesis with minimal systems
In: Physical Biology 16 (2019), Art.Nr.: 025001
ISSN: 1478-3967
DOI: 10.1088/1478-3975/aaf33d
Giordano G., De Graaf LM., Vasilakou E., Wahl SA.:
Unraveling energy homeostasis in a dynamic model of glycolysis in Escherichia coli
18th European Control Conference, ECC 2019 (Naples, 25. Juni 2019 - 28. Juni 2019)
In: 2019 18th European Control Conference, ECC 2019 2019
DOI: 10.23919/ECC.2019.8795884
Olavarria K., Fina A., Velasco MI., Van Loosdrecht MCM., Wahl SA.:
Metabolism of sucrose in a non-fermentative Escherichia coli under oxygen limitation
In: Applied Microbiology and Biotechnology 103 (2019), S. 6245-6256
ISSN: 0175-7598
DOI: 10.1007/s00253-019-09909-6
Straathof AJJ., Benjamin KR., Takors R., Wierckx N., Noorman HJ., Wahl SA.:
Grand Research Challenges for Sustainable Industrial Biotechnology
In: Trends in Biotechnology 37 (2019), S. 1042-1050
ISSN: 0167-7799
DOI: 10.1016/j.tibtech.2019.04.002
Zhang J., Martinez-Gomez K., Heinzle E., Wahl SA.:
Metabolic switches from quiescence to growth in synchronized Saccharomyces cerevisiae
In: Metabolomics 15 (2019), Art.Nr.: 121
ISSN: 1573-3882
DOI: 10.1007/s11306-019-1584-4

2017

Cueto-Rojas HF., Milne N., Van Helmond W., Pieterse MM., Van Maris AJA., Daran JM., Wahl SA.:
Membrane potential independent transport of NH3 in the absence of ammonium permeases in Saccharomyces cerevisiae
In: BMC Systems Biology 11 (2017), Art.Nr.: 49
ISSN: 1752-0509
DOI: 10.1186/s12918-016-0381-1

2016

Cueto-Rojas HF., Seifar RM., Ten Pierick A., Heijnen SJ., Wahl SA.:
Accurate measurement of the in vivo ammonium concentration in Saccharomyces cerevisiae
In: Metabolites 6 (2016), Art.Nr.: 12
ISSN: 2218-1989
DOI: 10.3390/metabo6020012
Milne N., van Maris AJ., Pronk JT., Daran JM., Wahl SA.:
Excessive by-product formation: A key contributor to low isobutanol yields of engineered Saccharomyces cerevisiae strains
In: Metabolic Engineering Communications 3 (2016), S. 39-51
ISSN: 2214-0301
DOI: 10.1016/j.meteno.2016.01.002
Niedenfuehr S., Ten Pierick A., Van Dam PTN., Suarez-Mendez CA., Noeh K., Wahl SA.:
Natural isotope correction of MS/MS measurements for metabolomics and 13C fluxomics
In: Biotechnology and Bioengineering 113 (2016), S. 1137-1147
ISSN: 0006-3592
DOI: 10.1002/bit.25859
Suarez-Mendez CA., Hanemaaijer M., ten Pierick A., Wolters JC., Heijnen JJ., Wahl SA.:
Interaction of storage carbohydrates and other cyclic fluxes with central metabolism: A quantitative approach by non-stationary 13C metabolic flux analysis
In: Metabolic Engineering Communications 3 (2016), S. 52-63
ISSN: 2214-0301
DOI: 10.1016/j.meteno.2016.01.001
Vasilakou E., Machado D., Theorell A., Rocha I., Noeh K., Oldiges M., Wahl SA.:
Current state and challenges for dynamic metabolic modeling
In: Current Opinion in Microbiology 33 (2016), S. 97-104
ISSN: 1369-5274
DOI: 10.1016/j.mib.2016.07.008

2015

Cueto-Rojas HF., Van Maris AJA., Heijnen JJ., Wahl SA.:
Thermodynamics-based design of microbial cell factories for anaerobic product formation
In: Trends in Biotechnology 33 (2015), S. 534-546
ISSN: 0167-7799
DOI: 10.1016/j.tibtech.2015.06.010
Milne N., Luttik MAH., Rojas HFC., Van Maris AJA., Pronk JT., Daran JM., Wahl SA.:
Functional expression of a heterologous nickel-dependent, ATP-independent urease in Saccharomyces cerevisiae
In: Metabolic Engineering 30 (2015), S. 130-140
ISSN: 1096-7176
DOI: 10.1016/j.ymben.2015.05.003
Willemsen AM., Hendrickx DM., Hoefsloot HCJ., Hendriks MMWB., Teusink B., Smilde AK., Van Kampen AHC., Wahl SA.:
MetDFBA: Incorporating time-resolved metabolomics measurements into dynamic flux balance analysis
In: Molecular bioSystems 11 (2015), S. 137-145
ISSN: 1742-2051
DOI: 10.1039/c4mb00510d
Zhang J., Sassen T., Ten Pierick A., Ras C., Heijnen JJ., Wahl SA.:
A fast sensor for in vivo quantification of cytosolic phosphate in Saccharomyces cerevisiae
In: Biotechnology and Bioengineering 112 (2015), S. 1033-1046
ISSN: 0006-3592
DOI: 10.1002/bit.25516
Zhang J., Ten Pierick A., Van Rossum HM., Seifar RM., Ras C., Daran JM., Heijnen JJ., Wahl SA.:
Determination of the Cytosolic NADPH/NADP Ratio in Saccharomyces cerevisiae using Shikimate Dehydrogenase as Sensor Reaction
In: Scientific Reports 5 (2015), Art.Nr.: 12846
ISSN: 2045-2322
DOI: 10.1038/srep12846

2014

De Jonge L., Buijs NAA., Heijnen JJ., Van Gulik WM., Abate A., Wahl SA.:
Flux response of glycolysis and storage metabolism during rapid feast/famine conditions in Penicillium chrysogenum using dynamic 13C labeling
In: Biotechnology Journal 9 (2014), S. 372-385
ISSN: 1860-6768
DOI: 10.1002/biot.201200260
Romagnoli G., Verhoeven MD., Mans R., Rey YF., Bel-Rhlid R., Van Den Broek M., Seifar RM., Ten Pierick A., Thompson M., Mueller V., Pronk JT., Daran JM., Wahl SA.:
An alternative, arginase-independent pathway for arginine metabolism in Kluyveromyces lactis involves guanidinobutyrase as a key enzyme
In: Molecular Microbiology 93 (2014), S. 369-389
ISSN: 0950-382X
DOI: 10.1111/mmi.12666
Van Heerden JH., Wortel MT., Bruggeman FJ., Heijnen JJ., Bollen YJM., Planque R., Hulshof J., O'Toole TG., Teusink B., Wahl SA.:
Lost in transition: Start-up of glycolysis yields subpopulations of nongrowing cells
In: Science 343 (2014), Art.Nr.: 1245114
ISSN: 0036-8075
DOI: 10.1126/science.1245114
Wahl SA.:
Flux response of glycolysis and storage metabolism during rapid feast/famine conditions in penicillium chrysogenum using dynamic 13C labeling
Metabolic Engineering X (Vancouver, BC, 15. Juni 2014 - 19. Juni 2014)
In: Metabolic Engineering X 2014
Wahl SA., Seifar RM., ten Pierick A., Ras C., van Dam JC., Heijnen JJ., van Gulik WM.:
Quantitative metabolomics using ID-MS
In: Jens O. Krömer, Lars K. Nielsen, Lars M. Blank (Hrsg.): Metabolic Flux Analysis, Humana Press Inc., 2014, S. 91-105 (Methods in Molecular Biology, Bd.1191)
DOI: 10.1007/978-1-4939-1170-7_6

2013

Jorda J., Suarez C., Carnicer M., Ten Pierick A., Heijnen JJ., Van Gulik W., Ferrer P., Albiol J., Wahl SA.:
Glucose-methanol co-utilization in Pichia pastoris studied by metabolomics and instationary 13C flux analysis
In: Bmc Systems Biology 7 (2013), Art.Nr.: 17
ISSN: 1752-0509
DOI: 10.1186/1752-0509-7-17

2012

Abate A., Hillen RC., Wahl SA.:
Piecewise affine approximations of fluxes and enzyme kinetics from in vivo 13C labeling experiments
In: International Journal of Robust and Nonlinear Control 22 (2012), S. 1120-1139
ISSN: 1049-8923
DOI: 10.1002/rnc.2798
Brinkman EK., Schipper K., Bongaerts N., Voges MJ., Abate A., Wahl SA.:
A toolkit to enable hydrocarbon conversion in aqueous environments
In: Journal of Visualized Experiments (2012)
ISSN: 1940-087X
DOI: 10.3791/4182
Cruz ALB., Hebly M., Duong GH., Pronk JT., Heijnen JJ., Daran-Lapujade P., Van Gulik WM., Wahl SA.:
Similar temperature dependencies of glycolytic enzymes: An evolutionary adaptation to temperature dynamics?
In: BMC Systems Biology 6 (2012), Art.Nr.: 151
ISSN: 1752-0509
DOI: 10.1186/1752-0509-6-151
Veiga T., Solis-Escalante D., Romagnoli G., Ten Pierick A., Hanemaaijer M., Deshmuhk A., Pronk JT., Daran JM., Wahl SA.:
Resolving phenylalanine metabolism sheds light on natural synthesis of penicillin G in Penicillium chrysogenum
In: Eukaryotic Cell 11 (2012), S. 238-249
ISSN: 1535-9778
DOI: 10.1128/EC.05285-11
Zhao Z., Ten Pierick A., De Jonge L., Heijnen JJ., Wahl SA.:
Substrate cycles in Penicillium chrysogenum quantified by isotopic non-stationary flux analysis
In: Microbial Cell Factories 11 (2012), Art.Nr.: 140
ISSN: 1475-2859
DOI: 10.1186/1475-2859-11-140

2011

Janke R., Genzel Y., Handel N., Reichl U., Wahl SA.:
Metabolic adaptation of MDCK cells to different growth conditions: Effects on catalytic activities of central metabolic enzymes
In: Biotechnology and Bioengineering 108 (2011), S. 2691-2704
ISSN: 0006-3592
DOI: 10.1002/bit.23215

2010

Janke R., Genzel Y., Freund S., Wolff MW., Grammel H., Ruehmkorf C., Seidemann J., Reichl U., Wahl SA.:
Expression, purification, and characterization of a His6-tagged glycerokinase from Pichia farinosa for enzymatic cycling assays in mammalian cells
In: Journal of Biotechnology 150 (2010), S. 396-403
ISSN: 0168-1656
DOI: 10.1016/j.jbiotec.2010.09.963
Janke R., Genzel Y., Reichl U., Wahl SA.:
Measurement of key metabolic enzyme activities in mammalian cells using rapid and sensitive microplate-based assays
In: Biotechnology and Bioengineering 107 (2010), S. 566-581
ISSN: 0006-3592
DOI: 10.1002/bit.22817
Ritter JB., Freund S., Genzel Y., Reichl U., Wahl SA.:
Metabolic effects of influenza virus infection in cultured animal cells: Intra- and extracellular metabolite profiling
In: Bmc Systems Biology 4 (2010), Art.Nr.: 61
ISSN: 1752-0509
DOI: 10.1186/1752-0509-4-61